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(a) Comparison of relative abundances and approximated complete abundances of dominant bacterial households in 114 fecal samples. The best panel demonstrates relative abundances depending on 16S amplicon sequencing and also the lessen panels shows the approximated complete abundances calculated by multiplying the relative abundances with full bacterial load, i.e. qPCR-primarily based estimate of copies of 16S gene for every one g of feces. (b) Correlation between the qPCR abundances (16S rRNA gene copies per g feces) and also the approximated complete abundances of 4 taxa representing species, genus, family and phylum stages. The dashed line exhibits the expected 1:1 correspondence.
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The use of relative abundance from following technology sequencing (NGS) data can lead to misinterpretations of microbial Group constructions as resulting from compositionality, the relative abundances from the taxa staying mutually dependent. This means that a rise of 1 taxon inevitably causes the concurrent decrease of one other(s). Considering that the changes of components are mutually dependent, significant Wrong discovery costs come about when compositional knowledge are analyzed employing conventional statistical procedures [1].
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The sequencing reads assigned to unique taxa in each sample ended up divided by the entire number of reads with the sample to get relative abundances from the taxa in Every sample. The relative abundances obtained according to the sequencing reads had been translated into full abundances by multiplying the relative abundance of each taxon by the entire bacterial abundance while in the sample.
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